Cat. No.
MABL-1787
Application
IP, neutralizing, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
C1
From
Recombinant Antibody
Specificity
This antibody is specific for the HDGF protein.
Alternative Names
Hepatoma-derived growth factor; High mobility group protein 1-like 2; HMG-1L2
UniProt
P51858
Immunogen
The original antibody was raised by immunizing mice with full-length recombinant HDGF. This antibody binds a different epitope on HDGF than antibody H3.
Application Notes
This antibody was used for immunoprecipitation on total cell lysates from H460 NSCLC cells (US20070243191). The neutralizing ability of this antibody was tested on A549. The result was a significant decrease in tumor size (US20070243191). This antibody was fluorescently labelled and injected into nude mice to test the antibody distribution. The antibodies evenly distributed in the animals including the tumors. This antibody entered into both cytoplasm and nuclear mainly in the tumor cells (US20070243191). Immunohistochemistry with this antibody was performed on tissues from NSCLC patients (US20070243191). NSCLC tissue was injected in mice and later harvested. This harvested tumor tissue was then used for immunohistochemistry with this antibody (Zhao et al, 2013; pmid:23695169). Western blot was performed on HDGF protein using this antibody (Ren et al, 2009;pmid:19435872).
Antibody First Published
Ren et al. Antibodies targeting hepatoma-derived growth factor as a novel strategy in treating lung cancer. Mol Cancer Ther. (2009); 8(5): 1106–1112. PMID:19435872
Note on publication
Describes the generation of anti-HDGF monoclonal antibodies and reports their anti- tumor activity in lung cancer xenograft models.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
