Anti-CD4 mAbs

Cat. No.

MABL-924

Name

Anti-CD4 mAbs

Clone No.

AFD- 3F11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, FACS, ELISA

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human CD4

Alternative Name

CD4mut; CD4 molecule; OKT4D; IMD79; 03F11;   CD4#8; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

UniProt

P01730

Immunogen

Application Notes

The binding of the original version of this   antibody (VHH) to CD4 was characterized in an ELISA. In a FACS binding assay,   this antibody exhibited an EC50 of 0.7 nM, 1.0 nM, and 0.76 nM for MOLM-13,   THP-1, and T cells, respectively, and in an HIV-1 neutralization it exhibited   an IC50 of 29.3 nM for PMBCs + NL4.3. This antibody was used to construct two   bi-specific nanobodies with clone 281F12 (Ab04369 Anti-CXCR4), one where it   was in the 1st position and one where it was in the 2nd position. Alone,it   bound CXCR4+/CD4+ MOLM-13 cells with a binding EC50 of 0.6 nM and   CXCR4+/CD4low Jurkat E6-1 cells with a binding EC50 of 61 nM. Its bi-specific   constructs bound the CXCR4+/CD4+ MOLM-13 and CXCR4+ /CD4low Jurkat E6-1 cells   with a binding EC50 of 1.1 and 11 nM when it was in the 2nd position and 0.7   and 1.1 nM when it was in the 1st position. 

Antibody First Published

Note on publication

The original publication describes the   construction and characterization of various bi- specific antibodies.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.