Key features and details | |
Cat. No. | MABL-925 |
Name | Anti-CD4 mAbs |
Clone No. | AFD- 6G5 (Zanolimumab) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC, Inhibit, |
Species Reactivity | chimpanzee, Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody binds human CD4. |
Alternative Name | Lu/T4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3 |
UniProt | P01730 |
Immunogen | The original antibody was generated by immunizing transgenic mice having functionally disrupted endogenous heavy chain loci (J.sub.HD) and harboring a human heavy chain minigene construct (HC1) with human blood from a single donor. |
Application Notes | Zanolimumab (clone 6G5, HuMax-CD4, MDX CD4, MDX-016) was among the first fully human mAbs generated from human Ig transgenic mice. The antigen specificity of this antibody was determined using ELISA (PMID: 9631008). The mAb 6G5 was subsequently re-expressed in CHO cells without altering its binding or functional characteristics and renamed HM6G. The avidity of 6G5 for native CD4 expressed on human and nonhuman primate lymphocytes was examined in a flow cytometry assay (PMID: 10444358). Zanolimumab inhibitsCD4+T cells by combining signaling inhibition with potent induction of Fc-mediated effector functions. T cell activation is inhibited by a fast dual mechanism in which the antibody abrogates signaling via the T cell receptor (TCR) and, in addition, down regulates T cell activation by transmission of direct inhibitory signals. In addition, zanolimumab induces killing of CD4+ T cells via antibody-dependent cellular cytotoxicity (PMID: 17942927). |
Antibody First Published | Fishwild et al. High-avidity human IgG kappa monoclonal antibodies from a novel strain of minilocus transgenic mice. Nat Biotechnol. (1996); 14(7):845-51. PMID:9631008 |
Note on publication | Describes the generation of humanized antibodies using transgenic mice. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
