Cat. No.
MABL-1809
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
4C11
From
Recombinant Antibody
Specificity
This antibody binds human hepcidin-25 in the N-terminus region. This antibody also cross reacts with mouse and rat hepcidin-2, to a much lesser degree, human hepcidin-22 but not human hepcidin- 20. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide stabilized by 4 disulfide bonds that plays a major role in innate immunity and iron homeostasis. It controls the delivery of iron to blood plasma from intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and from iron-storing hepatocytes. Hepcidin acts by binding to and inactivating the sole cellular iron exporter, ferroprotein, which delivers iron to plasma from all iron- transporting cells.
Alternative Names
HAMP; hHepc; HEPC; LEAP1; LEAP-1; Hepcidin; Liver-expressed antimicrobial peptide 1; hepcidin-22
UniProt
P81172
Immunogen
The original antibody was generated by immunizing mice with immunized with a N-terminal hepcidin peptide 'DTHFPIC' conjugated with a five amino acid peptide linker (i.e., GPGPG) to an OVA peptide (amino acids 323-336) sequence, i.e., full-length immunogen DTHFPICGPGPGISQAVHAAHAEINE.
Application Notes
This antibody can be used for the detection of human, rat or mouse hepcidin-25 using ELISA. This antibody can also detect human hepcidin-22 with a binding affinity of Kd= 209nM (US8609817).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
