Cat. No.
MABL-1820
Application
in vivo, SPR, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1A3
From
Recombinant Antibody
Specificity
The antibody is specific for human HGF.
Alternative Names
SF; Hepatocyte growth factor; Hepatopoietin-A; Scatter factor
UniProt
P14210
Immunogen
The original antibody was generated by immunizing Balb/c mice with recombinant human HGF.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis (IC50 = 5.65 nM). The binding affinity of the original format of the antibody to human HGF was measured by surface plasmon resonance (Kd= 30.1 pM). The antibody could bind to non-reduced and reduced rhHGF by western blot analysis. The antibody could efficiently neutralize HGF binding to c-Met. The antibody inhibited HGF induced proliferation in 4MBr-5 cells. The ability of the original format of the antibody to inhibit tumor growth was tested in an U87MG xenograft model. The percent of inhibition of the antibody was measured to be 73%. The ability of the original format of the antibody to inhibit tumor growth was tested in an U118 xenograft model. The percent of inhibition of the antibody was measured to be 57% (US7659378B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
