Cat. No.
MABL-1842
Application
IB, neutralization, ELISA
Isotype
Engineer antibody
Species Reactivity
Human immunodeficiency virus type 1
Clone No.
694-D
From
Recombinant Antibody
Specificity
This antibody is specific for the GRAF sequence of the gp120 V3 loop of HIV-1.
Alternative Names
Surface protein gp120; Envelope glycoprotein gp160; Env polyprotein
UniProt
P05877
Immunogen
The original antibody was generated by Epstein-Barr virus transformation of peripheral blood mononuclear cells from HIV-seropositive individuals and screening for reactivity against the rgp120IIIB peptide.
Application Notes
The original version of this antibody (human IgG1) was characterized for its specificity and reactivity using ELISA and IB assays. In the ELISA, it reacted with the V3 of HIV strains MN, SF-2, HXB2, RF, WM52, NY5, SC, and CDC4 with an affinity (Kd) of 1.8 µM and 12.0 µM for SF-2 and HXB2, respectively. In an IB, this antibody reacted with gp120HTLV-IIIB. In in vitro neutralization assays, this antibody exhibited neutralizing activity against multiple HIV-1 strains, exhibiting broad reactivity (US5914109A).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
