Cat. No.
MABL-1844
Application
Blocking, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
LG11
From
Recombinant Antibody
Specificity
This antibody recognises peptides corresponding to the tip of the MHC antigen-binding cavity (region 70 - 90) of HLA allele DQB1*0601 (RTRAELDTVCRHNYEVAFRGI). It cross-reacts with peptide DQB1*03/70–90, and the shorter peptide DQB1*06/70–79
Alternative Names
UniProt
Immunogen
This antibody was raised by immunising BALB/c mice with unconjugated free synthetic DQB1 peptides (region 70–90).
Application Notes
This antibody blocks the myasthenia gravis (MG)-associated DQ beta 1 allele, and is able to inhibit the proliferation of T cells from MG patients (Ayyar & Atassi, 2017). A humanised scFv derived from this antibody, which has been confirmed in ELISA analysis to recognise the same epitope, is also able to block the proliferation of T cells from MG patients typed DQB1*0601 (Ayyar & Atassi, 2017).
Antibody First Published
Oshima et al. Suppression by mAbs against DQB1 peptides of in vitro proliferation of AChR-specific T cells from myasthenia gravis patients Autoimmunity, 2005. 38:2, 161-169 PMID:16040337
Note on publication
Describes the original generation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
