Cat. No.
MABL-1885
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Mycobaterium bovis
Clone No.
G8
From
Recombinant Antibody
Specificity
This antibody is specific for the HSP65 protein from Mycobacterium bovis. Together with its co- chaperonin GroES, HSP65 plays an essential role in assisting protein folding. Mycobacterium bovis is the causative agent of tuberculosis in cattle.
Alternative Names
Chaperonin-60 2; Heat shock protein 65; Chaperonin GroEL 2; 60 kDa chaperonin 2; 65 kDa antigen; Antigen A; Cell wall protein A
UniProt
P0A521
Immunogen
This antibody was generated by panning a phage displayed repertoire derived from the immunoglobulin genes of the chicken against HSP65.
Application Notes
An ELISA was performed using the scFv version of this antibody on HSP65 to determine how efficiently the antibody can bind the antigen. Furthermore, an immunoblot was performed on HSP65 using this antibody (Wemmer et al, 2010; pmid:20299243).
Antibody First Published
Wemmer et al. Chicken scFvs and bivalent scFv-C(H) fusions directed against HSP65 of Mycobacterium bovis. Biologicals. 2010 May;38(3):407-14. PMID:20299243
Note on publication
Two chicken single-chain variable region antibody fragments (scFvs) that recognised the 65 kDa heat-shock protein (HSP65) of Mycobacterium bovis were selected from a large semi-synthetic phage displayed library. Both recognised HSP65 in indirect enzyme-linked immunosorbent assay (ELISA) and immunoblots and retained their activity during storage.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
