Cat. No.
MABL-1924
Application
inhibit, Block, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Clone 323
From
Recombinant Antibody
Specificity
This antibody binds human B7RP-1, which is a ligand for T-cell-specific cell surface receptor ICOS. It acts as a costimulatory signal for T-cell proliferation and cytokine secretion and also induces B-cell proliferation and differentiation into plasma cells.
Alternative Names
CD275; ICOSLG; ICOSL; B7-H2; B7H2; B7RP-1; B7RP1; KIAA0653; B7 homolog 2; B7-like protein GL50; B7-related protein 1
UniProt
O75144
Immunogen
The original antibody was isolated from a human naïve scFv phage library and panning against B7RP-1-Fc protein.
Application Notes
The scFv version of this antibody binds B7RP-1-Fc with a Kd= 9nM. The binding specificity of this antibody was confirmed using ELISA. This scFv version of this antibody was shown to bind B7RP-1-Fc in an ELISA. This antibody recognized B7RP-1 molecules expressed on B cells, as assessed by flow cytometry. This antibody is capable of inhibiting interaction between ICOS-Fc and B7RP-1-Fc. This antibody also exhibited inhibitory activity in a proliferation assay of T cells stimulated with anti-CD3 mAb and B7RP-1-Fc (PMID: 20065650).
Antibody First Published
Maeda et al. Regulation of T cell response by blocking the ICOS signal with the B7RP-1-specific small antibody fragment isolated from human antibody phage library. MAbs. (2009); 1(5): 453–461. PMID:20065650
Note on publication
Describes the generation of this antibody and its use in the study of T cell response by blocking the ICOS signal using this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
