Cat. No.
MABL-1926
Application
Block, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
HK5.3
From
Recombinant Antibody
Specificity
This antibody specifically binds to mouse ICOSL and competes with binding of ICOS.
Alternative Names
B7RP-1; B7-related protein 1; ICOS ligand; CD275; LICOS; B7h
UniProt
Q9JHJ8
Immunogen
This antibody was raised by immunizing SD rat with mouse B7h-transfected L cells and fusing immune splenocytes with P3U1 myeloma cells and screened for binding to mouse B7h-transfected NRK cells.
Application Notes
This antibody has been used to detect murine ICOSL by flow cytometry. It is able to block ICOSL interacting with ICOSL and inhibit T-cell proliferation in a collagen type II (CII)-induced arthritis model.
Antibody First Published
Iwai et al. Amelioration of collagen-induced arthritis by blockade of inducible costimulator-B7 homologous protein costimulation. J Immunol. 2002 Oct 15;169(8):4332-9 PMID:12370365
Note on publication
Describes the generation and initial characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
