Cat. No.
MABL-1928
Application
ADCC assays, CDC assays, Depletion, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
C2095
From
Recombinant Antibody
Specificity
C2095 targets the IdeS‐cleaved hinge of human IgG1, but not intact IgG1 or IgG1 cleaved by MMP-3 or GluV8. The antibody can also bind IdeS-cleaved hinge of human IgG4 but not IdeS-cleaved hinge of IgG2.
Alternative Names
IdeS-cleaved hinge of IgG4
UniProt
Immunogen
This antibody was raised by immunising rabbit with a KLH‐conjugated peptide (CTAPPAPAPELLG).
Application Notes
ELISA demonstrated C2095 has robust binding to IdeS-generated F(ab')2 fragments of human IgG1 and human IgG4 but does not bind IdeS-generated F(ab')2 fragments of human IgG2. It also does not bind the intact IgG1, IgG2 or IgG4 counterparts (Brezski et al, 2014). ADCC and CDC assays showed C2095 is capable of restoring antibody-dependent cell-mediated cytotoxicity and complement- dependent cytotoxicity against WIL2-S cells to the otherwise inactive anti-CD20 IgG1 F(ab’)2 fragment. It also reinstated ADCC against MDA-MB-231 cells to an anti-CD142 IgG1 F(ab’)2 fragment. This antibody was also capable of eliciting both CDC and ADCC to IgG4 F(ab’)2 fragments (Brezski et al, 2014).
Antibody First Published
Brezski et al, 2014. A monoclonal antibody against hinge-cleaved IgG restores effector function to proteolytically-inactivated IgGs in vitro and in vivo. MAbs. 2014;6(5):1265-73 PMID:25517311
Note on publication
Describes the original generation of C2095 and its characterisation through ADCC and FCC assays.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
