Cat. No.
MABL-1935
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
8C5F8
From
Recombinant Antibody
Specificity
This antibody specifically binds human interferon gamma, which is a type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation.
Alternative Names
IFN-γ; IFNG; IFN-gamma; Interferon gamma; Immune interferon
UniProt
P01579
Immunogen
The original antibody was generated by immunizing mice with human interferon-gamma protein.
Application Notes
This antibody specifically binds human interferon gamma in an ELISA and does not cross react with IL-2, IL-4, IL-6, IL-10, GM-CSF and TNF-alpha. The original mouse IgG version of this antibody binds human IFN-gamma with a binding affinity of Kd= 41.1 pM. A combination of antibodies 8C5F8 and 13E6H4 can be used in a double antibody sandwich ELISA, where 8C5F8 is used as a capture antibody and 13E6H4 is used as a detection antibody (CN116284381).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
