Cat. No.
MABL-1940
Application
neutralizing, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Chicken
Clone No.
YC1
From
Recombinant Antibody
Specificity
The antibody binds specifically to IFN-gamma, a dimerized soluble cytokine and the only member of the type II class of interferon.
Alternative Names
Interferon gamma; Interferon-gamma; IFN-gamma; IFNG; IFN-g; Type II interferon
UniProt
P49708
Immunogen
The original antibody was raised by immunizing BALB/c mice with recombinant chicken IFN-y.
Application Notes
The specificity of this antibody (IgG2b) was confirmed by ELISA analysis. The IFN-g ELISA was also capable of measuring cytokine levels in the sera of chickens orally infected with Eimeria maxima. The antibody detected the recombinant IFN-y by western blot analysis. The antibody was capable of neutralizing IFN-g bioactivity against virus-infected cells (Yun et al., 2000; pmid: 10713342).
Antibody First Published
Yun et al. Chicken IFN-gamma monoclonal antibodies and their application in enzyme‐linked immunosorbent assay. Vet I mmunol Immunopathol. 2000;73(3‐4):297‐308. PMID:10713342
Note on publication
The paper describes the generation and characterization of the antibody, specific for chicken IFN-y for the purpose of developing an IFN-y-specific enzyme-linked immunosorbent assay.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
