Cat. No.
MABL-1950
Application
Blocking, in vitro, in vivo, therapeutic
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
rhuMAb-E25
From
Recombinant Antibody
Specificity
This antibody is specific for the Fc region of human IgE.
Alternative Names
Immunoglobulin epsilon; FcERI; FCER1; FCϵRI; FCϵR1; Fc-epsilon RI-alpha; IgE Fc receptor subunit alpha
UniProt
P12319
Immunogen
The original antibody is the recombinant humanized version of MaE11, a murine anti-IgE monoclonal antibody, constructed from the complementarity determining regions of MaE11 and a human IgG1 frame.
Application Notes
This antibody has two potential binding sites on IgE (one for each Fc heavy chain) and, therefore, can function as a multivalent ligand that complexes with IgE in various conformations depending on the ratio of their molar concentrations (Liu et al., 1995; PMID: 7654701). This antibody has been shown to reduce serum IgE levels and significantly attenuate both the early- and late-phase responses to inhaled allergen in allergic asthmatic subjects (Fahy et al., 1997; PMID: 9196082). Omalizumab treatment has been shown to reduce asthma exacerbations and decrease inhaled corticosteroid and rescue medication use in the treatment of severe allergic asthma (Busse et al., 2001; PMID: 11496232). The therapeutic product made using this antibody (omalizumab) binds to human IgE in blood or membrane-bound IgE on B lymphocytes. Furthermore, omalizumab competes with IgE's high and low-affinity receptors — FcεRI and CD23, respectively — for binding to residues in the CH3 domain. Therefore, it effectively blocks human IgE binding to FcϵRI without stimulating the degranulation of mast cells and basophils, i.e., without triggering a severe, life-threatening anaphylactic reaction (Wright et al., 2015; PMID: 26113483).
Antibody First Published
Liu et al. Characterization of complex formation by humanized anti-IgE monoclonal antibody and monoclonal human IgE Biochemistry. 1995 Aug 22;34(33):10474-82. doi: 10.1021/bi00033a020 PMID:7654701
Note on publication
The original publication characterizes the formation of immune complexes between a humanized anti-IgE monoclonal antibody and IgE, revealing various complex structures and a high-affinity interaction.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
