Cat. No.
MABL-1958
Application
inhibitory, IP
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
IGFR 24-57
From
Recombinant Antibody
Specificity
This antibody is specific for the human insulin-like growth factor 1 receptor (IGF1R). It recognises the extracellular portion of the receptor, specifically an epitope in the alpha subunit (amino acids 440-514). It does not cross-react with rat or mouse IGF1R or human insulin receptors in co-precipitation or immunodepletion assays, but does react with rabbit IGFR.
Alternative Names
CD221; IGF1 receptor; Insulin-like growth factor 1 receptor; type I IGF receptor; Insulin-like growth factor I receptor; IGF-I receptor; 24 57
UniProt
P08069
Immunogen
This antibody was raised by immunising BALB/c mice with transfected mouse fibroblasts expressing high levels of human IGF1R (IGF1R/3T3).
Application Notes
The ability of this antibody to bind human IGF1R has been confirmed in IGF1 binding assays using both IG1FR-overexpressing IGF1R/3T3 cells and soluble receptors (Soos et al, 1992). This antibody efficiently precipitates IGF1R solubilized from IGF1R/3T3 cells and receptor-IGF1 complexes (Soos et al, 1992). When immobilised, this antibody has been shown to remove essentially all soluble IGF1R in immunodepletion experiments (Soos et al, 1992). This antibody strongly inhibits IGF1 binding to its receptor (Soos et al, 1992), and has been shown to suppress IGF1-mediated stimulation of skin fibroblast proliferation (Okubo et al 2003).
Antibody First Published
Soos et al. A panel of monoclonal antibodies for the type I insulin-like growth factor receptor. Epitope mapping, effects on ligand binding, and biological activity. J Biol Chem. 1992 Jun 25;267(18):12955-63. PMID:1377676
Note on publication
Describes the original generation of this antibody, and the characterisation of its specificity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
