Cat. No.
MABL-1975
Application
dot blot assay, ICC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
TP886
From
Recombinant Antibody
Specificity
This antibody recognises Fab fragment of mouse IgG1 (both with kappa and lambda light chains).
Alternative Names
immunoglobulin G1 antigen-binding fragment kappa light chain; immunoglobulin G1 antigen-binding fragment lambda light chain; kappa light chain; lambda light chain; immunoglobulin G1
UniProt
Immunogen
This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
Application Notes
TP886 nanobody can be used as an efficient high-affinity reagent for immunofluorescence. It was utilised in conjugation with two to three fluorophores in detection of mouse monoclonal IgG1 for the indirect immunostaining of HeLa cells where it performed exceptionally well as for a non-polyclonal antibody reaching similar signal strength to polyclonal agents when it was combined with another anti-IgG1 nanobody (Pleiner et al., 2018). Monovalent nanobodies can be also effectively used in a single step immunostaining procedure because they do not create large, cross-linked complexes with primary antibodies.TP886 was used as a secondary reagent; it was first incubated with a primary antibody and then a one-step immunostaining was performed (without the usual separate incubation with a primary and secondary antibody (Pleiner et al., 2018).
Antibody First Published
Pleiner et al. A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. PMID:29263082
Note on publication
The article describes the generation and characterisation of this nanobody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
