Cat. No.
MABL-1980
Application
dot blot assay, IP
Isotype
Engineer antibody
Species Reactivity
Rat (weak), Mouse
Clone No.
TP881
From
Recombinant Antibody
Specificity
This antibody recognises Fc fragment of the IgG1.
Alternative Names
immunoglobulin G1 fragment crystallizable region; immunoglobulin G1 Fc region; immunoglobulin G1
UniProt
Immunogen
This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
Application Notes
TP881 nanobody can be used for the purification of antigens or antigen complexes previously bound to a primary antibody, as it successfully isolated mouse monoclonal IgG1 from hybridoma cell culture supernatant and then released it under physiological conditions using SUMOStar protease cleavage (Pleiner et al., 2018).
Antibody First Published
Pleiner et al. A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. PMID:29263082
Note on publication
The article describes the generation and characterisation of this nanobody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
