Cat. No.
MABL-1984
Application
dot blot assay
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
TP894
From
Recombinant Antibody
Specificity
This antibody recognises Fc fragment of the IgG1.
Alternative Names
immunoglobulin G1 fragment crystallizable region; immunoglobulin G1 Fc region; immunoglobulin G1
UniProt
Immunogen
This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
Application Notes
Anti-IgG nanobodies often perform more efficiently than polyclonal secondary antibodies in various experimental assays. Therefore, they are recommended for various experimental procedures. Anti-IgG nanobodies were shown to be more sensitive during Western blots when conjugated with different molecules (HRP, fluorophores etc.) (Pleiner et al., 2018). Furthermore, combination of two distinct nanobodies for immunofluorescence labelling provided exceptionally clean and strong signal, unprecedented among monovalent secondary antibodies (Pleiner et al., 2018). Additionally, using such an antibody allows skipping two-step incubation procedures during immunostaing as the nanobody can be first incubated with a primary antibody and this conjugate might be added to the cells in a one-step procedure (Pleiner et al., 2018). Finally, such nanobodies have been recommended as very effective for colocalisation studies when more than one fluorophore is needed (Pleiner et al., 2018).
Antibody First Published
Pleiner et al. A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. PMID:29263082
Note on publication
The article describes the generation and characterisation of this nanobody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
