Cat. No.
MABL-1993
Application
dot blot assay, ICC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
TP1129
From
Recombinant Antibody
Specificity
This antibody recognises Fc region of mouse IgG2a.
Alternative Names
immunoglobulin G2a fragment crystallizable region
UniProt
Immunogen
This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
Application Notes
TP1129 can be used as an efficient secondary nanobody in the indirect immunofluorescence. It was utilised to detect IgG2a primary antibodies alongside another nanobody giving strong, clean signal without any background staining (Pleiner et al., 2018).
Antibody First Published
Pleiner et al. A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar 5;217(3)：1143-1154. doi： 10.1083/jcb.201709115. Epub 2017 Dec 20. PMID：29263082
Note on publication
The article describes the generation and characterisation of this nanobody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
