Cat. No.
MABL-2011
Application
IP, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Elasmobranch
Clone No.
GA8
From
Recombinant Antibody
Specificity
This antibody is specific for IgNAR, an Ig heavy chain isotype specific to elasmobranchs, and reacts with an epitope in the constant domain. This anti-nurse shark IgNAR antibody has been shown to cross-react with spiny dogfish IgNAR.
Alternative Names
Shark HC antibody; Immunoglobulin new antigen receptor; immunoglobulin novel antigen receptor; novel antigen receptor; NAR; Q8AXH5; Q90544
UniProt
P83977
Immunogen
This antibody was raised by immunising mice with nurse shark IgNAR.
Application Notes
This antibody immunoprecipitates IgNAR from both nurse shark and spiny dogfish plasma (Smith et al, 2012), and from serum and yolk extracts from mature unfertilized nurse shark eggs (Haines et al, 2005). This antibody has been used in immunohistochemical analysis of IgNAR expression in frozen sections of nurse shark spleen, to identify its cellular organisation (Rumfelt et al, 2002). This antibody has been used in ELISA analysis to measure plasma titres of IgNAR following spiny dogfish immunisation with human serum albumin (Muller et al, 2012) and nurse shark immunisation with human and murine induced costimulatory ligand (Kovaleva et al, 2017) and with recombinant human TNF-α (Ubah et al, 2017).
Antibody First Published
Rumfelt et al. The Development of Primary and Secondary Lymphoid Tissues in the Nurse Shark Ginglymostoma cirratum: B‐Cell Zones Precede Dendritic Cell Immigration and T‐Cell Zone Formation During Ontogeny of the Spleen Scand J Immunol. 2002 Aug;56(2):130-48. PMID:12121433
Note on publication
Describes the original use of this antibody in immunohistochemical analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
