Key features and details | |
Cat. No. | MABL-946 |
Name | Anti-CD40 mAbs |
Clone No. | AFD- FGK45 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Activating, agonist, functional assays, WB, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody recognizes CD40. It is a type I transmembrane glycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD40 is expressed broadly on antigen-presenting cells (APCs) such as dendritic cells, B cells, macrophages, and monocytes as well as non-immune endothelial cells, basal epithelial cells, and a range of tumors. Upon binding to its ligand CD154, CD40 acts as a costimulatory molecule for the activation of B cells, dendritic cells, monocytes, and other APCs. |
Alternative Name | Tnfrsf5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; FGK4.5 |
UniProt | P27512 |
Immunogen | The original rat IgG2a antibody was raised against the recombinant mouse CD40 fusion protein. |
Application Notes | FGK45 is an agonistic antibody that has been shown to activate CD40 expressing antigen presenting cells (APCs) what might promote anti-tumor T cell responses. For instance, the rat IgG2a version of this antibody was used to promote maturation of APCs what improved the ability of the macrophages to fight a tumour (Luheshi et al., 2016; pmid: 26918344). Another group used the same antibody to stimulate T-cells in a study on a tumor vaccine (Bartkowiak et al., 2015; pmid: 26351680).Others used ths clone as well to induce potent anti-tumor activity (Carmi et al., 2015; pmid: 25924063). On the other hand, it can also be used to inhibit CD40/CD154 interaction in vitro and in vivo. Furthermore, this clone can be also utilized to identify CD40-positive cells by flow cytometry (Rolink et al., 1996; pmid: 8885865). Administering this antibody can be also utilized to induce inflamatory bowel disease-like characteristics and create a mouse IBD model to study this class of diseases (Bauche et al., 2018; pmid: 30097293). |
Antibody First Published | Rolink et al. The SCID but not the RAG-2 gene product is required for S mu- S epsilon heavy chain class switching Immunity. 1996 Oct;5(4):319-30. doi: 10.1016/s1074-7613(00)80258-7. PMID:8885865 |
Note on publication | Describes the generation and characterization of the antibody FGK45. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
