Key features and details | |
Cat. No. | MABL-947 |
Name | Anti-CD40 mAbs |
Clone No. | AFD- G28.5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Activate, IP, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | 48 kD Type I glycoprotein, a member of the TNFR superfamily primarily expressed on normal or malignant B cells or B cell lines, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. |
Alternative Name | TNFRS5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor CDw40; CD154R |
UniProt | P25942 |
Immunogen | Human erythrocyte-rosette-negative tonsillar lymphocytes. |
Application Notes | This antibody detects CD40 by immunostaining. The original mouse IgG1 format was shown to stimulate B-cells (Bishop 2012 and references therein). |
Antibody First Published | Clark EA, Ledbetter JA. Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4494-8. PMID:3487090 |
Note on publication | Describes the making of the antibody, shows it recognised a 50-kDa polypeptide expressed on all B cells using immunoprecipitation and flow cytometry. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
