Cat. No.
MABL-2039
Application
Blocking, therapeutic, ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
MM17F3
From
Recombinant Antibody
Specificity
This antibody is highly specific for murine IL-17A; in ELISA analysis, no cross-reactivity with IL‐17B, IL‐17C, IL‐17D, IL‐17E or IL‐17F was observed.
Alternative Names
Interleukin-17A; IL17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8
UniProt
Q62386
Immunogen
This antibody was raised by immunising C57Bl/6 mice with IL-17A cross-linked to ovalbumin to generate a highly immunogenic complex.
Application Notes
The specificity of this antibody has been confirmed in ELISA analysis (Uyttenhove et al, 2006). This antibody inhibits IL‐17A‐induced IL‐6 production by a Cl1D fibroblastic cell line in vitro (Uyttenhove et al, 2006). This antibody has been shown to prevent experimental autoimmune encephalomyelitis (EAE) disease development in vivo (Uyttenhove et al, 2006), as well as protecting C57Bl/6 mice from myelin oligdendrocyte glycoprotein (MOG)-induced EAE (Uyttenhove et al, 2007). Additionally, administration of this antibody inhibits collagen-induced arthritis (CIA) in IFN-γ receptor knockout mice (Kelchtermans et al, 2009).
Antibody First Published
Uyttenhove et al. Development of an anti‐IL‐17A auto‐vaccine that prevents experimental auto‐immune encephalomyelitis Eur J Immunol. 2006 Nov;36(11):2868-74 PMID:17048276
Note on publication
Describes the original generation of this antibody, and the characterisation of its IL- 17A-inhibitory activity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
