Cat. No.
MABL-2047
Application
functional assay, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
18F4.2C8
From
Recombinant Antibody
Specificity
This antibody binds human Interleukin-1 alpha, which is a cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells.
Alternative Names
IL1A; IL-1α; IL1F1; Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1
UniProt
P01583
Immunogen
The original antibody was generated by immunizing mice with recombinant human IL-1 alpha.
Application Notes
The original mouse IgG antibody binds human IL-1A with a binding affinity of Kd= 0.6 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 8.90E−11M. This antibody was used in combination with 1B12.4H4 (anti-IL-1β) antibody for the generation of anti-human IL-1α/β DVD-Ig molecule that had therapeutic potential (PMID: 20068402).
Antibody First Published
Wu et al. Molecular construction and optimization of anti-human IL-1α/β dual variable domain immunoglobulin (DVD-Ig™) molecules. MAbs. 2009 Jul-Aug; 1(4): 339–347. PMID:20068402
Note on publication
Describes the generation of anti-human IL-1α/β DVD-Ig™ molecules using several pairs of monoclonal antibodies with therapeutic potential.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
