Key features and details | |
Cat. No. | MABL-949 |
Name | Anti-CD40 mAbs |
Clone No. | AFD- LOB7/6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, IP, SPR, FC, IHC |
Species Reactivity | Dog, Human |
Basic Information | |
Specificity | This antibody is specific for human CD40, a costimulatory protein on the surface of antigen presenting cells. This antibody has been shown to be cross-reactive with canine CD40. |
Alternative Name | TNFSR5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor CDw40; CD154R |
UniProt | P25942 |
Immunogen | |
Application Notes | This antibody was used for immunohistochemistry (IHC) of post-mortem brain tissues. In all tissues examined, the blood vessels were stained positively for CD40 (Togo et al., 2000; PMID: 11121537). This antibody was used for flow cytometry (FC) of endothelial cell lines (Lidington et al., 1999; PMID: 10638837), dendritic cells (Urban et al., 1999; PMID: 10403251), and peripheral blood B cells and monocytes (Fan et al., 2012; PMID: 22459705). This antibody was found to be the optimal antibody for CD40 marking in an FC assay of the antibodies tested (LOB7/6, 82105, 11E9, and a rabbit polyclonal antibody). Furthermore, the effect of its binding to CD40 on keratinocyte proliferation and apoptotic rate was investigated; it was found not to inhibit keratinocyte proliferation. Rather, it had the opposite effect, as it enabled the keratinocytes to retain their proliferative potential and did not induce apoptosis (Villarroel Dorrego et al., 2006; PMID: 16487107). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
