Cat. No.
MABL-2074
Application
FACS, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
57A07
From
Recombinant Antibody
Specificity
This antibody is specific for the alpha subunit of IL-3R
Alternative Names
CD123; IL3R; IL3RAY; IL3RX; IL3RY; hIL-3Ra; interleukin 3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha
UniProt
P26951
Immunogen
Application Notes
The binding of the original version of this antibody (VHH) to IL3-Ra-expressing viral lipoparticles was characterized in an ELISA (KD 0.783 nM). In a FACS binding assay, this antibody exhibited an EC50 of 0.66 nM, 1.3 nM, and 0.24 nM for MOLM-13, THP-1, and HEK-IL-R3a, respectively, and an IC50 of 1.20 nM in competition against clone 7G3. In its bi-specific constructions with clones 14D09 and 281F12, it served as an anchor (US20220242958).
Antibody First Published
Note on publication
The original publication describes the construction and characterization of various bi- specific antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
