Cat. No.
MABL-2086
Application
neutralising, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2F2
From
Recombinant Antibody
Specificity
This antibody is specific for human rIL-5, and does not cross-react with human rIL-1α, rIL-lβ, rIL- 4, rIL-8, rM-CSF, or rTGF-α.
Alternative Names
interleukin 5; IL5; IL 5; Eosinophil differentiation factor
UniProt
C9JQP9
Immunogen
This antibody was raised by immunising CAF1 mice with human recombinant IL-5.
Application Notes
This antibody has been show to inhibit the binding of human rIL-5 to the alpha chain of the IL-5 receptor (Ames et al, 1995). This antibody inhibits the proliferation of murine B13 cells in response to human IL-5 (Ames et al, 1995).Neutralising antibodies against IL-5 represent a potential therapeutic strategy for the treatment of eosinophilia. The specificity of this antibody for human IL-5 has been confirmed in ELISA analysis (Ames et al, 1995).
Antibody First Published
Ames et al. Neutralizing murine monoclonal antibodies to human IL-5 isolated from hybridomas and a filamentous phage Fab display library. J. Immunol. 154 (12), 6355-6364 (1995) PMID:7759873
Note on publication
Describes the original generation of this antibody, and its characterisation and use in ELISA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
