Cat. No.
MABL-2112
Application
Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
M36-11
From
Recombinant Antibody
Specificity
This antibody binds human interleukin-8. Interleukin-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
Alternative Names
IL8; Interleukin-8; CXCL8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor; 4-123-36
UniProt
P10145
Immunogen
The original antibody 4-123-36 was isolated from a human single domain antibody (scFv) library by screening the library against extracellular portions of the human IL-8. Affinity maturation of this clone using mutagenesis by error-prone PCR resulted in the generation of M36-11.
Application Notes
The binding of this affinity-maturated antibody to hIL8 was analyzed using ELISA. This antibody is capable of blocking the biological activity of human interleukin 8 at a concentration of 1 and 10 μg/mL in a neutrophil chemotaxis assay (PMID: 20718039).
Antibody First Published
Ding et al. Generation of high-affinity fully human anti-interleukin-8 antibodies from its cDNA by two-hybrid screening and affinity maturation in yeast. Protein Sci. 2010 Oct; 19(10): 1957–1966. PMID:20718039
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
