Cat. No.
MABL-2143
Application
in vitro, in vivo, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BBBB138
From
Recombinant Antibody
Specificity
The antibody is specific for the insulin receptor. The antibody recognises both short isoform (IR- A) and a long isoform (IR-B). The epitope of the antibody does not overlap with the insulin binding site.
Alternative Names
IR
UniProt
P06213
Immunogen
The original antibody was isolated from a human phage display library by panning against IR.
Application Notes
The specificity of the antibody for IR was determined by ELISA analysis. The binding affinity of the antibody to IR-A and IR-B was measured by surface plasmon resonance (Kd= 2.57 and 2.98 nM respectively). The antibody blocked insulin induced IR phosphorylation in a dose-dependent manner on HuH7 cells. The antibody effectively decreased insulin induced glucose uptake at concentrations of 1 nM and 10 nM in vitro. The antibody induced hyperglycemia in C57 mice in in vivo experiments.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
