Cat. No.
MABL-2146
Application
crystallization
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Fab 83-7
From
Recombinant Antibody
Specificity
This antibody recognizes an epitope within the cysteine rich region (amino acids 140-301) of the extracellular domain of the human insulin receptor alpha.
Alternative Names
CD220
UniProt
P06213
Immunogen
This antibody was raised by immunizing mice with IM-9 lymphocytes followed by purified insulin receptor.
Application Notes
This antibody forms a stable complex with the L1-CR fragment of the human insulin receptor alpha-subunit. Consequently, this antibody can be used to facilitate the crystallization of the complex, and the establishment of its structure (McKern et al, 2006).
Antibody First Published
McKern et al. Structure of the insulin receptor ectodomain reveals a folded- over conformation Nature, 2006, Vol.443(7108), p.218 PMID:16957736
Note on publication
Describes the original generation of Fab fragments for Mab 83-7, and their subsequent use in the crystallization of insulin receptor alpha.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
