Cat. No.
MABL-2151
Application
Cristallography, in vitro, in vivo, IP, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
TS2/16
From
Recombinant Antibody
Specificity
The antibody is specific for human integrin alpha 6 beta 1. It does not cross-react with mouse integrin β1.
Alternative Names
CD29; Integrin α6β1; Integrin alpha-6/beta-1 (ITGA6:ITGB1); Integrin alpha VI beta 1; ITGA6; CD49F; Integrin alpha-6; CD49 antigen-like family member F; VLA-6; ITGB1; FNRB; MDF2; MSK12; Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta
UniProt
P23229; P05556
Immunogen
Application Notes
The antibody immunoprecipitated the 130 kDa ß1 form of in U87MG cells. Flow cytometry analysis performed with the antibody confirmed that it could recognise ß1-A172 cells (Bartik et al., 2008; PMID: 18425328). The structure of the Fv-clasp fragment of the antibody was determined. Further, the crystal structure of the a6b1 headpiece complexed with Fv-clasp was determined (Arimori et al., 2017; PMID: 28919443). The antibody increased integrin ß1 in vitro and in vivo (Ritsma et al., 2017; PMID: 28448494)
Antibody First Published
Hemler et al. Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines. J Immunol June 1, 1984, 132 (6) 3011-3018; PMID:6327814
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
