Cat. No.
MABL-2177
Application
crystallization, SPR
Isotype
Engineer antibody
Species Reactivity
Leishmania tarentolae
Clone No.
Nb6
From
Recombinant Antibody
Specificity
This antibody is specific for J-base binding protein 1 (JBP1), a protein that contributes to the biosynthesis and maintenance of base J (β-d-glucosylhydroxymethyluracil), a modification of thymidine confined to some protozoa.
Alternative Names
J-binding protein 1; J-base binding protein 1; Thymine dioxygenase JBP1
UniProt
Q9U6M1
Immunogen
Application Notes
Surface plasmon resonance screening identified this antibody as a strong binder,recognizing JBP1 with a 1:1 stoichiometry and high affinity (Kd = 30 nM) (van Beusekom et al., 2018; PMID: 30387773).
Antibody First Published
van Beusekom et al. Characterization and structure determination of a llama-derived nanobody targeting the J-base binding protein 1 Acta Crystallogr F Struct Biol Commun. 2018 Nov 1;74(Pt 11):690-695. doi: 10.1107/S2053230X18010282 PMID:30387773
Note on publication
The original publication describes the characterization and structure determination of a llama-derived nanobody that targets the J-base binding protein 1 (JBP1), which is involved in the biosynthesis and maintenance of base J, a thymidine modification found in certain protozoa.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
