Cat. No.
MABL-2180
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
5B5
From
Recombinant Antibody
Specificity
The antibody is specific for Jo-1. Histidyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, it catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Jo-1 represents a key autoantibody target in the anti-synthetase syndrome that is marked by myositis as well as extra-muscular organ complications including interstitial lung disease (ILD).
Alternative Names
histidyl tRNA synthetase; Histidine--tRNA ligase, cytoplasmic; HisRS
UniProt
P12081
Immunogen
The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected Jo-1 by western blot analysis (Hof et al., 2008; PMID: 18062792).
Antibody First Published
Hof et al. Multiple-antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens Clin Exp Immunol. 2008 Feb;151(2):367-77. PMID:18062792
Note on publication
Describes the generation of chicked antibodies against various human autoantigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
