Cat. No.
MABL-2184
Application
WB
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
NH3/41.34
From
Recombinant Antibody
Specificity
This antibody recognises human immunoglobulin kappa light chain. The antibody does not cross react with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4.
Alternative Names
kappa Lc; human immunoglobulin kappa light chain; kappa chain; immunoglobulin kappa light chain; NH3/41
UniProt
Immunogen
This antibody was raised by immunising Balb/c mice with whole IgG fraction and subsequent hybridoma cells formation.
Application Notes
NH3/41.34 antibody detects kappa light chains from a human IgGs and Bence-Jones protein samples via Western blot under reducing conditions (Thorpe et al., 1984). This antibody is equally able to detect full IgGs via Western blot when the samples are transferred from the non-reducing SDS polyacrylamide gels (Thorpe et al., 1984). Further, this antibody was capable of detecting IgGs via Western blot from native gels run under acidic conditions (Thorne et al., 1984). Finally, the specificity of the antibody for human IgG1 was confirmed by ELISA analysis. No cross reactivity with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4 was detected.
Antibody First Published
The use of monoclonal antibodies to human IgG in blood transfusion serology. Biotest Bulletin 4: 348-352 (1983)
Note on publication
This article describes the generation and characterisation of the NH3/41.34 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
