Cat. No.
MABL-2187
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Streptomyces lividans
Clone No.
uysa_K-2_Fab4
From
Recombinant Antibody
Specificity
This antibody is specific for the last 30 residues of KscA (Uysal et al, 2009; pmid: 19346472).
Alternative Names
SKC1; Streptomyces lividans K+ channel; FAb4; Fab 4; pH-gated potassium channel KcsA
UniProt
P0A334
Immunogen
The original Fab version of this antibody was obtained by recombinant antibody phage display.
Application Notes
ELISA was preformed with this antibody demonstrating its binding it to KscA. This was done to research the structure of the KscA protein (Uysal et al, 2009; pmid:19346472).
Antibody First Published
Uysal et al. Crystal structure of full-length KcsA in its closed conformation Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6644-9. PMID:19346472
Note on publication
Synthetic antigen-binding fragments (Fabs) were used as crystallographic chaperones to determine the structure of full-length KcsA at 3.8 A, as well as that of its isolated C-terminal domain at 2.6 A.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
