Key features and details | |
Cat. No. | MABL-967 |
Name | Anti-CD44 mAbs |
Clone No. | AFD- Hermes-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, ICC, IP, neutralization, WB, ELISA, FC, IF, IHC |
Species Reactivity | Bovine, Goat, Primate, Rabbit, Rat, Sheep, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for CD44 (gp90 epitope cluster I), which is selectively expressed on normal or transformed lymphoid cells that are able to recognize and bind to high endothelial venules (specialized vessels that mediate lymphocyte exit from the blood into lymphoid organs). Although this antibody was raised against human CD44, it cross-reacts with murine, rat, rabbit, primate, sheep, bovine and goat CD44. |
Alternative Name | CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; CD44/H-CAM antibody; Hermes 1 |
UniProt | P16070 |
Immunogen | This antibody was produced by immunizing a Fischer rat with human tonsil lymphocytes (10^7cells intraperitoneally in Freund's complete adjuvant). A boost was given 2 weeks later (intravascularly in saline). |
Application Notes | This antibody binds CD44, so can be used to label CD44+ cells in flow cytometry analysis. As CD44 molecules are used to bind to high endothelial venules (HEV) at the site of lymphoctye entry to lymphoid organs, this antibody can be used to specifically stain HEV-binding cell lines (Jalkanen, 1986). In addition, this antibody blocks the adhesion of the haematopoietic progenitor cell line KG1 to hyaluronic acid (Bendall, 2003), but does not block lymphocyte binding to HEV (Picker, 1989). 7H9D6 partially blocks the binding of this antibody. |
Antibody First Published | Jalkanen et al. A lymphoid cell surface glycoprotein involved in endothelial cell recognition and lymphocyte homing in man. Eur J Immunol. 1986 Oct;16(10):1195-202. PMID:2429846 |
Note on publication | Describes the original use of this antibody in preliminary attempts to characterise its ligand (CD44), using IHC-fr, IP and IF. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
