Cat. No.
MABL-2236
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
PL60B3
From
Recombinant Antibody
Specificity
This antibody recognizes human and mouse laminin 211 LG5 domain. Laminin is a large extracellular heterotrimeric glycoprotein and a major constituent of the basement membrane. It is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin-211 (formerly named merosin) is the most abundant laminin isoform in the basement membrane of adult skeletal muscle. Laminin-211 deficiency leads to the most common form of congenital muscular dystrophy in childhood, MDC1A.
Alternative Names
Merosin; Laminin subunit alpha-2; LAMA2; Laminin 211; laminin G-like 5 domain; LG5; g-like 4 and 5 domain; LG-4/5; Laminin M chain; Laminin-12 subunit alpha; Laminin-2 subunit alpha; Laminin-4 subunit alpha; Merosin heavy chain
UniProt
P24043; Q60675
Immunogen
The original antibody was generated by immunizing an alpaca with a mixture of transfected CHO cells expressing human and mouse laminin 211 LG5 domain.
Application Notes
The binding specificity of this antibody to human and mouse laminin 211 LG5 expressed on the surface of CHO cells was confirmed using ELISA and flow cytometry. The binding affinity of the original VHH antibody to human beta dystroglycan was determined to be 68 pM. This antibody was used in combination with anti-beta dystroglycan antibody P38G1 for the generation of a bispecific antibody (CN113651891).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
