Cat. No.
MABL-2261
Application
crystallography, ICC, neutralizing, SPR, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Bovine, Cat, Chicken, Guinea Pig, Rat, Human
Clone No.
5D2
From
Recombinant Antibody
Specificity
The antibody is specific for lipoprotein lipase (LPL) and it binds to a tryptophan (Trp)-rich loop in the carboxyl terminus of LPL. The antibody does not cross react with mouse LPL. LPL is a key enzyme in triglyceride metabolism, it catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage.
Alternative Names
LPL; Phospholipase A1; EC 3.1.1.34
UniProt
P06858
Immunogen
The original antibody was generated by immunizing mice with bovine LPL.
Application Notes
LPL was immunoprecipitated from postheparin plasma using this antibody. The antibody was used both to capture LPL and to detect the bound LPL in a sandwich ELISA, indicating the epitope to be present in duplicate. In another sandwich ELISA analysis, the antibody was used to detect the LPL antigen (Peterson et al., 1992, PMID: 1279089). The antibody detected LPL from human, chicken and guinea pig by western blot analysis. The specificity of the original format of the antibody was confirmed by ELISA analysis (Chang et al., 1998; PMID: 9831623). Pretreatment of LPL with the antibody totally suppressed LPL-induced monocyte adhesion to endothelial cells (Mamputu et al., 1997; PMID: 9323582). Further, LDL was immunoneutralized with the antibody, totally suppressing LPL-induced VSMC proliferation (Mamputu et al., 2000; PMID: 11031206).
Antibody First Published
Peterson et al. Human lipoprotein lipase: relationship of activity, heparin affinity, and conformation as studied with monoclonal antibodies J Lipid Res. 1992 Aug;33(8):1165-70.PMID:1279089
Note on publication
The paper describes the generation and characterization of the antibody. The antibody is used to investigate how conformational changes in lipoprotein lipase affect its molecular functions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
