Cat. No.
MABL-2291
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BAR-1
From
Recombinant Antibody
Specificity
This antibody is specific for Lys–amidine-GM3g.
Alternative Names
ganglioside GM3
UniProt
Immunogen
The original antibody was raised by immunizing a mouse with HEL-[–X-GM3g]6 LOG antigens.
Application Notes
This antibody was used for an ELISA on ganglioside GM3. This antibody was used for surface plasmon resonance on C(NH)NH)-GM3g. The structure of this antibody was determined using x-ray crystallography (Deimel et al., 2023).
Antibody First Published
Deimel et al. Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response biorxiv
Note on publication
The original paper used protein editing methods to display GM3g in systematically varied non-native presentation modes by attachment to carrier protein lysine sidechains using diverse chemical linkers to probe the limits of glycan tolerance.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
