Cat. No.
MABL-2306
Application
FACS, inhibition, ELISA
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
Caline
From
Recombinant Antibody
Specificity
This antibody is specific for human M-CSF. It has been shown to cross-react with cynomolgus monkey M-CSF. M-CSF is a secreted cytokine that influences hematopoietic stem cells to differentiate into macrophages or other related cell types.
Alternative Names
MCSF; CSF1; Macrophage colony-stimulating factor; Macrophage colony- stimulating factor 1; Lanimostim; Proteoglycan macrophage colony-stimulating factor 1; PG-M-CSF 1
UniProt
P09603
Immunogen
The original antibody was selected from a commercially available phage display library. The library was panned against the receptor-binding domain of human M-CSF (rbdM-CSF).
Application Notes
The original version of this antibody (human IgG1) was tested for its specificity for human M-CSF and cross-reactivity for cynomolgus M-CSF using ELISA. KD values of 14 pM and 16 pM were measured. This antibody binds M-CSF expressed on cells, as shown in a FACS assay that exhibited an EC50 value of 9.8 nM and 14 nM against M-CSF-expressing transfected CHO cells and MDA-MB 231 cells, respectively. This antibody successfully inhibited human and cynomolgus M-CSF binding to recombinant human M-CSF receptor with an IC50 of 6.1 pM and 6.3 pM, respectively. This antibody successfully inhibited the proliferation of M-NFS-60 cells induced by recombinant and native human and cynomolgus M-CSF with an IC50 of 3.1 pM and 17.7 pM for recombinant and native human M-CSF, respectively, and 4.3 pM and 106.9 pM for recombinant and native cynomolgus M-CSF, respectively. This antibody successfully inhibited the proliferation of M-NFS-60 cells induced by membrane-bound human M-CSF (expressed by stably transfected CHO cells) (US10081675B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
