Cat. No.
MABL-2317
Application
Block, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
MECA-89
From
Recombinant Antibody
Specificity
The antibody is specific for mouse MAdCAM-1. The antibody recognizes the second immunoglobulin domain, interfering with L-selectin interactions of MAdCAM-1. The mucosal lymphocyte homing receptor MAdCAM-1 is expressed in the high endothelial venules of the mesenteric lymph nodes (MLN) and Peyer's patches (PP) and in the flat epithelium of the lamina propria (LP).
Alternative Names
ATCC HB-292; Addressin; mucosal vascular addressin cell adhesion molecule 1
UniProt
Immunogen
Endothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes.
Application Notes
The antibody bound to the second domain of MAdCAM-1 and did not block MAdCAM-1- dependent binding in vitro (Streeter et al., 1988; PMID:3340147). The antibody was used for immunohistochemical analysis of healthy AKR control mice and it showed faint basal expression of MAdCAM- 1 in the terminal ileum (Bachmann et al. 2006). The antibody blocked interactions with L-selectin in vivo (Bargatze et al., 1995; PMID: 7542550).
Antibody First Published
Streeter et al. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Nature. 1988 Jan 7;331(6151):41-6. PMID:3340147
Note on publication
The original paper describes the role of MAdCAM-1 in lymphocyte homing.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
