Cat. No.
MABL-2328
Application
flow cytometry, IP, WB, IF, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
PLK1
From
Recombinant Antibody
Specificity
This antibody recognises the extracellular domain V between residues 420 and 431 in MARCO both in human and bovine samples.
Alternative Names
SCARA2; macrophage receptor MARCO; macrophage receptor with collagenous structure; scavenger receptor class A member 2; PLK-1; PLK 1
UniProt
Q9UEW3
Immunogen
The antibody was raised by immunising BALB/c mice with human alveolar macrophages (AMs) and subsequent creation of hybridomas.
Application Notes
The specificity of this antibody was confirmed in an IF experiment where the antibody stained alveolar macrophages for MACRO (Arredouani et al., 2005). Tissue samples prepared in acetone and paraformaldehyde were also successfully immunohistochemically stained (Arredouani et al., 2005; Martens et al., 2006). The antibody was also capable of weak staining of alveolar macrophages in flow cytometry (Palecanda and Kobzik, 2001). In IP experiments, the antibody immunoprecipitates MARCO from lysates obtained from COS cells transfected with human MARCO (Arredouani et al., 2005).
Antibody First Published
Arredouani et al. MARCO Is the Major Binding Receptor for Unopsonized Particles and Bacteria on Human Alveolar Macrophages J Immunol November 1, 2005, 175 (9) 6058-6064 PMID:16237101
Note on publication
The article describes the method of generation of anti-MARCO PKL1 antibody and its effectivenes in IHC, IP and functional experiments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
