Cat. No.
MABL-2332
Application
ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
C3A8
From
Recombinant Antibody
Specificity
This antibody binds an epitope found on the MCF-7 breast carcinoma cell line.
Alternative Names
MCF-7; MCF7; breast cancer cells
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with the MCF-7 breast carcinoma cell line.
Application Notes
The original IgM version of this antibody strongly reacted with breast cancer cells (MCF7) and colon cancer cells (HT29) in an ELISA. Immunofluorescence staining suggested that the antigen this antibody detected was located in the cytoplasm of MCF7 and HT29 cell lines but there was no positive staining detected on cervical cancer (HeLa) and fibroblast normal cells (3T3). The original IgM version of the antibody detected a 55 kDa protein that was present in the extract of MCF7 and HT29 cell lines in a western blot assay (Abdullah & Ali., 2006). The scFv version of this antibody (B7) also showed high reactivity towards MCF-7 breast cancer cells in an ELISA (PMID: 24301925). The binding reactivity of the scFv version of this antibody was also tested using flow cytometry and it was suggested that the antigen was expressed in periplasmic space of MCF-7 cells when tested using an annexin V-based apoptosis assay (PMID: 25022376).
Antibody First Published
Zuhaida et al. Construction of single-chain variable fragment antibodies against MCF-7 breast cancer cells. Genet Mol Res. 2013 Nov 18;12(4):5547-59. PMID:24301925
Note on publication
Describes the generation of an scFv version of the original mouse IgM antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
