Cat. No.
MABL-2338
Application
IHC;, IP, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
A103
From
Recombinant Antibody
Specificity
This antibody binds human Melan-A protein. This protein is found on the surface of melanocytes and is an important marker for detection of melanocytic tumors.
Alternative Names
MART-1; MART1; Melan A; MLANA; Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A; Melanoma antigen recognized by T-cells 1; MALENA
UniProt
Q16655
Immunogen
The original antibody was generated by subcutaneously immunizing BALB/c mice with recombinant Melan-A protein in complete Freund's adjuvant.
Application Notes
The binding characterization of this antibody towards Melan-A was done using ELISA. The original mouse IgG1 antibody was able to detect Melan-A from cell lysates of SK-MEL-13, SK-MEL-23, and SK-MEL-28 cell lines in an immunoblot assay. This antibody was also able to immunoprecipitate Melan-A protein from cell lysates of SK-MEL-13, SK-MEL-23, and SK-MEL-28 cell lines. The original mouse antibody was also used in the immunohistochemical analysis of normal skin and two Melan-A positive melanomas SK- MEL-19 and MZ2-MEL3.1 (PMID: 8650193). This antibody was used for detection of Melan-A antigen on formalin-fixed, paraffin-embedded tissues and in diagnosis of metastatic melanoma (PMID: 9591730). Immunohistochemical analysis with this antibody was useful in the differential diagnosis of adrenocortical neoplasms from both renal cell and hepatocellular carcinomas (PMID: 14663359; 9422316). This antibody has also been explored in the differential diagnosis of ovarian sex cord stromal tumours using immunohistochemistry (PMID: 10823140).
Antibody First Published
Chen et al. Serological analysis of Melan-A(MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5915-9. PMID:8650193
Note on publication
Describes the generation of this antibody and mentions its use in the characterization of the antigen (Melan-A) that it binds.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
