Cat. No.
MABL-2340
Application
immunotherapy, radioimmunotherapy, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
8C3
From
Recombinant Antibody
Specificity
This antibody binds melanin. Melanin is a natural pigment found in most organisms is produced in a specialized group of cells called the melanocytes.
Alternative Names
CHEBI:89634
UniProt
Immunogen
The original antibody was generated by immunizing female BALB/c mice with melanin particles extracted from Paracoccidioides brasiliensis L-DOPA-induced yeast cells suspended in a 1:1 (vol/vol) emulsion of incomplete Freund adjuvant.
Application Notes
Paracoccidioides brasiliensis is the agent of the human systemic disease paracoccidioidomycosis. The binding characterization of this antibody towards melanin from various organisms was done using ELISA. This antibody was also used in the immunofluorescence analysis of P. brasiliensis melanized yeast cells (with l-DOPA induction) and wild-type conidia (PMID: 21813659). 213Bismuth and 177Lutetium labelled humanized 8C3 antibody was used in the radioimmunotherapy (RIT) of experimental B16-F10 melanoma (PMID: 31323785). It was demonstrated that a combination of humanized anti-melanin antibody conjugated to 213Bismuth and anti-PD-1 ICB reduced tumor growth and increased survival in the Cloudman S91 murine melanoma DBA/2 mouse model (PMID: 31991626). Male Cloudman S91-bearing DBA/2 mice when treated intraperitoneally with a combination of anti-PD-1 alone or low-dose humanized anti-melanin antibody conjugated to177Lutetium RIT alone resulted in modest tumor reduction, while their combination significantly reduced tumor growth and increased survival, suggesting synergy (PMID: 33218169).
Antibody First Published
Urán et al. Detection of antibodies against Paracoccidioides brasiliensis melanin in in vitro and in vivo studies during infection Clin Vaccine Immunol. 2011 Oct;18(10):1680-8. PMID:21813659
Note on publication
Describes the generation and characterization of antibodies towards melanin particles from P. brasiliensis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
