Cat. No.
MABL-2355
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
AD3
From
Recombinant Antibody
Specificity
Alternative Names
MDI
UniProt
Immunogen
Mouse serum protein conjugated with MDI.
Application Notes
The antibody binds specifically to MDI, a low-molecular weight aromatic diisocyanate which is commonly used to make polyurethane. MDI is also a major cause of occupational asthma, due to its ability to sensitize the immune system and elicit an immune response. In humans, the carrier protein albumin is able to recognise and conjugate with MID, forming a complex which is bound by IgE, triggering an asthmatic attack. Monoclonal antibodies directed against MID can be applied to diagnostics and research,and can be used to detect and quantitate MID levels in blood and urine, as well as in environmental samples. Monoclonal antibodies AD3 and AH6 bind to similar or overlapping epitopes.
Antibody First Published
Wisnewski AV, Liu J. Molecular determinants of humoral immune specificity for the occupational allergen, methylene diphenyl diisocyanate. Mol Immunol. 2013 Jun;54(2):233-7. PMID:23295252
Note on publication
Describes the production of six monoclonal antibodies directed against MDI, which could be developed into useful diagnostic reagents.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
