Cat. No.
MABL-2383
Application
ICC, tumor imaging, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
COU-1
From
Recombinant Antibody
Specificity
This antibody binds modified forms of human cytokeratin 8 and cytokeratin 18. It is reported that this antibody recognizes an intracellularly located cytokeratin protein that is strongly expressed in adenocarcinomas.
Alternative Names
KRT8; CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; Type-II keratin Kb8; KRT18; CYK18; Keratin type I cytoskeletal 18; Cytokeratin-18; Cell proliferation-inducing gene 46 protein; COU1; B9165; ECACC 87040201
UniProt
P05787; P05783
Immunogen
The original antibody was generated by fusing the human lymphoblastoid cell line WI-L2-729- HF2 with lymphocytes obtained from mesenteric lymph nodes from a colon cancer patient.
Application Notes
This antibody reacted with a panel of colon cancer cell lines and melanoma cell lines in a immunocytochemical assay. This antibody strongly reacted with antigens expressed on autologous and allogeneic colorectal cancers in an immunohistochemical analysis of formalin-fixed paraffin-embedded tissues (PMID: 3699931). Electron microscopy of colonic adenocarcinoma cells, intact tumor and colonic epithelium by the immunogold technique demonstrated that the C-OU1 antibody reacted with a molecule associated with areas of disruption of the intermediate filaments in the cytoplasm of the tumor cells (PMID: 1607210). This binding of this antibody to different recombinant heterotypic K8/K18 complexes was determined by ELISA. This antibody cannot bind K8(1-65)/intact K18 and intact K8(1-85)/intact K18 complexes in an ELISA (US20050048070).
Antibody First Published
Christensen et al. Human-human hybridomas for the study of anti-tumor immune response in patients with colorectal cancer. Int J Cancer. 1986 May 15;37(5)；683-8. PMID:3699931
Note on publication
Describes the generation of a human-human hybridoma with lymphocytes from patients with colorectal cancer.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
