Cat. No.
MABL-2399
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
A5 scFv
From
Recombinant Antibody
Specificity
A5 scFv binds specifically to the extracellular N-terminus of MRP1, with no cross-reactivity with other MRPs or BCRP. FC analysis showed that A5 reacts strongly with viable ovarian carcinoma 2008/MRP1 and small cell lung carcinoma GLC4/ADR cells that overexpress MRP1. MRP1 is a member of the ABC superfamily of transporter proteins and confers resistance to various toxic compounds and are involved in multi-drug resisitance in chemotherapy. This protein mediates the export of organic anions and drugs from the cytoplasm.
Alternative Names
LTC4 transporter; Multidrug resistance-associated protein 1; MRP-1; MRPI; MRP-I; ATP-binding cassette sub-family C member 1; Leukotriene C(4) transporter; LTC4 transporter
UniProt
P33527
Immunogen
A5 scFv was isolated from a phage display. The source of the antibody genes came from the spleens of mice which had been immunized with f88 filamentous bacteriophages displaying a peptide derived from the extracellular N-terminus (aa 1-18) of human MRP1. The scFv A5 was expressed in BL21 E. coli cells and purified from the periplasmic fraction by metal-ion affinity chromatography.
Application Notes
standard and saturation ELISA. The avidity of the scFv wsa increased for FC studies through tetramerization of A5 by adding a BirA tag to the C terminus for site-specific biotinylation and tetramerized using PE-labelled streptavidin.
Antibody First Published
Binyamin et al. Targeting an extracellular epitope of the human multidrug resistance protein 1 (MRP1) in malignant cells with a novel recombinant single chain Fv antibody. Int J Cancer. 2004 Jul 20;110(6)；882-90. PMID：15170671
Note on publication
Describes the generation and characterization of A5 scFv, which binds specifically to MRP1.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
