Cat. No.
MABL-2403
Application
functional assay, FC
Isotype
Engineer antibody
Species Reactivity
Monkey, Human
Clone No.
LC007
From
Recombinant Antibody
Specificity
This antibody is specific for repeat 14 of MSCP or for an epitope that is only reconstituted when repeat 14 is present. It also binds to cynomolgus MSCP. MSCP is a mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe2+, is then probably delivered to ferrochelatase to catalyze Fe2+ incorporation into protoprophyrin IX to make heme (By similarity).
Alternative Names
Mitoferrin-1; SLC25A37; Mitochondrial iron transporter 1; Mitochondrial solute carrier protein; Solute carrier family 25 member 37
UniProt
Q9NYZ2
Immunogen
The original mouse antibody was raised by immunizing Balb/c mice with a synthetic peptide corresponding to aa 2177-2221 of the human MCSP sequence coupled to KLH.
Application Notes
The binding epitope of this antibody was mapped by preforming flow cytometry on 4 different constructs. These were: construct 1 containing CSPG repeat 15, construct 2 containing CSPG repeat 14-15, construct 3 containing CSPG repeat 13-15, and construct 4 containing CSPG repeat 12-15 (US2014242083). Flow cytomtery was used to determine whether the human version of this antibody has cross-reactivity with human and cynomolgus antigen. The cells used were HEK-EBNA expressing either a construct containing the C-terminal part of the cynomolgus MCSP protein, a signal peptide for secretion and a N-terminal FLAG-tag or the human variant of this construct (US2014242083). Further, lysis of Colo38 human malignant melanoma cells by human lymphocytes was assessed using the glycoengineerd mouse version of this antibody (US2014242083). Co-culturing CD16-CAR T cells with A375 cells in presence of the human version of this antibody resulted in a dose-dependent increase in activation for the human version of this antibody (Rataj et al, 2018; pmid:30429531).
Antibody First Published
Rataj et al. High-affinity CD16-polymorphism and Fc-engineered antibodies enable activity of CD16-chimeric antigen receptor-modified T cells for cancer therapy Br J Cancer. 2019 Jan;120(1):79-87. PMID:30429531
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
