Cat. No.
MABL-2407
Application
ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
Nb61
From
Recombinant Antibody
Specificity
The original antibody exhibits selective reactivity for misfolded SOD1 proteins (SOD1 G93A, SODox and SOD1 A4V).
Alternative Names
SOD1 G93A; SODox; SOD1 A4V
UniProt
Immunogen
The original antibody was generated by immunizing llamas simultaneously with recombinant SOD1 WT, SOD1 G93A, and an oxidized form of SOD1 (SODox), followed by library construction. The antibody was isolated by panning against SODox and counterscreened to exclude clones that exhibit high reactivity for the native SOD1 WT protein.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis, the antibody exhibited selectivity for human mutant SOD1 (SOD1 G93A, SODox and SOD1 A4V) over murine WT SOD1. The original format of the antibody enhanced SOD1-myc signal intensities in HEK293T cells. The original format of the antibody did not reduce expression levels of misfolded SOD1 protein in mammalian cells, but appeared to stabilize the misfolded conformation of mutant SOD1 in cells and in vitro. As measured by differential scanning fluorimetry, the complex formation between the original format of the antibody and SOD1 A4V have a stabilizing effect on the mutant protein. The group performed studies in ALS-relevant models including human iPSC-derived motor neurons harboring the SOD1 A4V mutation. The original format of the antibody exhibited selectivity for this mutant protein. Transduction of lentiviruses expressing the original format of the antibody and the antibody linked to PEST were not toxic to human neurons but rather conferred a beneficial effect on the health of SOD1 A4V neurons. Both neurite outgrowth and SOD1 levels were enhanced upon expression of the original format of the antibody compared to the control condition (Kumar et al., 2022; PMID: 36555655).
Antibody First Published
Kumar et al. Anti-SOD1 Nanobodies That Stabilize Misfolded SOD1 Proteins Also Promote Neurite Outgrowth in Mutant SOD1 Human Neurons Int J Mol Sci. 2022 Dec 16;23(24):16013. doi: 10.3390/ijms232416013 PMID:36555655
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
